Background
see Certificate of Analysis
- Catalogue Number
- 60-0122-500
- Product Size
- 500 µl
- Price £
- £265
- Certificate of Analysis Size
- 500 µl
- Species
- human
- Source
- synthetic
- Quantity
- 500 µl
- Storage
- -70°C
- Concentration
- 0.4µM
- Formulation
- DTT containing buffer
- Molecular Weight
- ~8.93kDa
- Stability
- 12 months at -70°C. Avoid multiple freeze/thaw cycles.
- Protein Sequence
- Accession number: P62987. C-terminally tagged with Rhodamine 110
- QA; Protein Identification
- Confirmed by mass spectrometry.
- QA Activity
Activity Assay: The activity of Ubiquitin-Rhodamine 110 was validated by determining the increase in fluorescence at 535nm (Excitation 485nm) measured as a result of the enzyme catalysed cleavage at the amide bond between the C-terminal Glycine and Rhodamine, generating Ubiquitin and de-quenched Rhodamine 110-Glycine. UCHL3 (deubiquitylase) was incubated with Ubiquitin-Rhodamine 110 and the fluorescence was measured at four time points (0min, 30min, 60min and 90min).